The PredictorTM hERG Fluorescence Polarization Assay Implementation on Tecan ́s Infinite F500 Multimode Reader
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چکیده
FP is based on the principle that when a small fluorescent molecule (the tracer) is excited with plane-polarized light, the emitted light is largely depolarized because the molecule rotates rapidly in solution during its fluorescence lifetime. If the tracer is bound to a large molecule, thereby increasing its molecular volume, its rotation is slowed and the emitted light remains in the same plane as it was excited [1]. Invitrogen developed a FP based assay to perform hERG (human Ether-a-go-go Related Gene) channel biochemical binding studies displacing radiometric binding assays [2]. PredictorTM hERG FP Assay
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PredictorTM hERG Fluorescence Polarization Assay Kit performed on the PHERAstar/PHERAstar Plus
The potential for cardiotoxic side-effects continues to challenge the development of small molecule based therapies. These intolerable side-effects are often precipitated by drug-induced long QT syndrome (LQT), which is often linked to blocking the human ether-a-go-go related gene (hERG) potassium channel. Although patch-clamp electrophysiology remains the gold standard for determining the inte...
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تاریخ انتشار 2008